Amy Keating<span>, PhD</span>
Karl Berggren<span>, PhD</span>

Amy Keating, PhD and Karl Berggren, PhD

Departments of Biology and Biological Engineering
/ Awarded in 2017

Brick by Brick

Creating a whole new class of nano-bio building blocks
Premise

A multidisciplinary approach to a biomolecular problem

Living organisms arrange proteins and DNA into intricate systems that accomplish wide-ranging sensing, information-processing, mechanical, and chemical functions. These naturally occurring biological designs are difficult for engineers to match in complexity and efficiency. If they could, these biomolecular systems could have broad applicability and functionality in materials, computing, and other applications. “Unfortunately,” says Dr. Amy Keating, “current methods are slow and costly. We need to learn how to synthesize giant user-defined biomolecular systems with the complexity of integrated circuit patterns.”

Combining her expertise in biological engineering with that of her collaborator, electrical engineer Dr. Karl Berggren, the pair conceived a means to develop methods and tools to construct complex, nano-scale assemblies of biological molecules on surfaces. The project leverages each of the labs’ capabilities; applying the two disciplines to a single objective is novel and promises to take their work farther than any previous efforts. The chief responsibility of Dr. Keating’s lab is to design custom protein molecules while Dr. Berggren’s lab will be focused on making patterned substrates with specific features through electron-beam lithography—together, they seek to construct a unique nano-bio interface design platform.

Protein engineering enables bottom-up design, while lithography provides top-down templating; we believe the two can be combined to create new types of materials,” says Dr. Keating. “Such a framework could power discovery and engineering in the life, materials, and information-processing sciences, and enable new types of nano-manufacturing.”

Bringing together specialists in nanopatterning and protein assembly to solve the problem of protein positioning represents a radically new approach and the insertion of substantially new expertise.”

Challenge

Taking a broad, long-term view turns off traditional funding sources

The interdisciplinary nature of this approach (combining electrical engineering, materials science, and protein biology) is necessary, but is difficult to accommodate in traditional funding mechanisms. The pair previously obtained some support for this type of work from a major global chemical and life sciences company, but there was pressure to steer the work towards near-term applications. “That was in conflict with our goals of creating new basic capabilities to support a broad range of as-yet not imagined applications,” says Dr. Keating.

Our goals are to make new parts, develop new methods, optimize processes, explore opportunities, and identify limitations,” Dr. Berggren adds. “These goals are too basic for most industry funding.”

Potential

Building on early successes, hopeful for lasting impact

The pair plan to introduce additional complexity into their systems by designing a broader range of structures, diversifying their toolkit of surface attachment strategies, and aligning protein structures to more complex surface patterns. Ultimately, the goal of their research is to engineer solutions such that the surface can effectively direct the organization of the proteins into larger, programmed complexes and assemblies.